Evaluation of Pharmacological Properties of Curcuma zedoaria Rhizome via Ethnopharmacological Approaches
DOI:
https://doi.org/10.69728/jst.v10.37Keywords:
Curcuma zedoaria, Methanolic extract, Hepatoprotective, Antibacterial, CytotoxicityAbstract
Phytochemicals or plant secondary metabolites secure plant cells in stressful circumstances and offer human well-being and hence are used in traditional medicine. Curcuma zedoaria (Christm.) Roscoe of the family Zingiberaceae has numerous traditional uses, however its pharmacological effectiveness has not yet been fully scrutinized. This study intended to explore the pharmacological properties of the plant using the crude methanolic extract (ME) of its rhizome. In vitro and in vivo assessments were performed to ascertain the hepatoprotective, antimicrobial, and cytotoxic effects. A promising dose-dependent hepatoprotective effect was observed in paracetamol (2 g/kg body weight) induced hepatotoxic rats, pretreated with the ME (150 & 300 mg/kg body weight). There was a significant decline (p<0.001) in the level of the serum biomarkers of hepatic necrosis (Alanine Aminotransferase, Alkaline Phosphatase, Aspartate Aminotransferase, and Total Bilirubin). Antibacterial potency of the ME was evaluated against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus employing the disc diffusion method, where a dose of 800 µg demonstrated notable effects (p<0.01) compared to the reference standards, Kanamycin and Norfloxacin. Finally, a mild dose-dependent response was observed compared to the reference standard vincristine sulfate in in vitro brine shrimp lethality bioassay, a test taken as an indicator of cytotoxicity. To conclude, the findings of this study opened an avenue for more extensive research to find new phytochemicals with hepatoprotective, antibacterial, and anticancer potential.
References
Banisalam, B., Sani, W., Philip, K., Imdadul, H., & Khorasani, A. (2011). Comparison between in vitro and in vivo antibacterial activity of Curcuma zedoaria from Malaysia. African Journal of Biotechnology, 10(55), 11676–11681.
Bantawa, P., & Rai, R. (2009). Studies on ethnomedicinal plants used by traditional practitioners, Jhankri, Bijuwa and Phedangma in Darjeeling Himalaya. Natural Product Radiance, 8(5), 537–541.
Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians, 68(6), 394–424.
Brüssow, H. (2024). The antibiotic resistance crisis and the development of new antibiotics. Microbial Biotechnology, 17(7), e14510.
Chachad, D. P., Talpade, M. B., & Jagdale, S. P. (2016). Antimicrobial activity of rhizomes of Curcuma zedoaria Rosc. International Journal of Science and Research, 5(11), 938–940.
Chen, W., Lu, Y., Gao, M., Wu, J., Wang, A., & Shi, R. (2011). Anti-angiogenesis effect of essential oil from Curcuma zedoaria in vitro and in vivo. Journal of Ethnopharmacology, 133(1), 220–226.
Devarbhavi, H., Asrani, S. K., Arab, J. P., Nartey, Y. A., Pose, E., & Kamath, P. S. (2023). Global burden of liver disease: 2023 update. Journal of hepatology, 79(2), 516–537.
Dhal, Y., Sahu, R. K., & Deo, B. (2011). Ethnomedicinal survey of Koraput district, Odisha: An update. Journal of Pharmacy Research, 4(11), 4142–4145.
Elekofehinti, O. O., Adanlawo, I. G., Komolafe, K., & Ejelonu, O. C. (2012). Saponins from Solanum anguivi fruits exhibit antioxidant potential in Wistar rats. Annals of Biological Research, 3(7), 3212–3217.
Farzaei, M. H., Zobeiri, M., Parvizi, F., El-Senduny, F. F., Marmouzi, I., Coy-Barrera, E., Naseri, R., Nabavi, S. M., Rahimi, R., & Abdollahi, M. (2018). Curcumin in Liver Diseases: A Systematic Review of the Cellular Mechanisms of Oxidative Stress and Clinical Perspective. Nutrients, 10(7), 855.
Fried, M. W. (2002). Side effects of therapy of hepatitis C and their management. Hepatology, 36(S1), S237–S244.
Gharge, S., Hiremath, S. I., Kagawad, P., Jivaje, K., Palled, M. S., & Suryawanshi, S. S. (2021). Curcuma zedoaria Rosc (Zingiberaceae): a review on its chemical, pharmacological and biological activities. Future Journal of Pharmaceutical Sciences, 7(166), 1–9.
Han, K. L., Jung, M. H., Sohn, J. H., & Hwang, J. (2006). Ginsenoside 20S-protopanaxatriol (PPT) activates peroxisome proliferator-activated receptor gamma (PPARgamma) in 3T3-L1 adipocytes. Biological and Pharmaceutical Bulletin, 29(1), 110–113.
Islam, M., Hoshen, M. A., Islam, F., & Yeasmin, T. (2017). Antimicrobial, membrane stabilizing and thrombolytic activities of ethanolic extract of Curcuma zedoaria Rosc. Rhizome. Journal of Pharmacognosy and Phytochemistry, 6(5), 38–41.
Jin, P., Jiang, J., Zhou, L., Huang, Z., Nice, E. C., Huang, C., & Fu, L. (2022). Mitochondrial adaptation in cancer drug resistance: prevalence, mechanisms, and management. Journal of Hematology & Oncology, 15(97), 1–42.
Karim, M. S., Rahman, M. M., Shahid, S. B., Malek, I., Rahman, M. A., Jahan, S., Jahan, F. I., & Rahmatullah, M. (2011). Medicinal plants used by the folk medicinal practitioners of Bangladesh: a randomized survey in a village of Narayanganj district. American-Eurasian Journal of Sustainable Agriculture, 5(4), 405–414.
Kanase, V., & Khan, F. (2018). An overview of medicinal value of Curcuma species. Asian Journal of Pharmaceutical and Clinical Research, 11(2), 40–45.
Kumar, G., Banu, G. S., Pappa, P. V., Sundararajan, M., & Pandian, M. R. (2004). Hepatoprotective activity of Trianthema portulacastrum L. against paracetamol and thioacetamide intoxication in albino rats. Journal of Ethnopharmacology, 92(1), 37–40.
Kumarasamy, K. K., Toleman, M. A., Walsh, T. R., Bagaria, J., Butt, F., Balakrishnan, R., … Woodford, N. (2010). Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: A molecular, biological, and epidemiological study. The Lancet Infectious Diseases, 10(9), 597–602.
Kyung, E. J., Kim, H. B., Hwang, E. S., Lee, S., Choi, B. K., Kim, J. W., Kim, H. J., Lim, S. M., Kwon, O. I., & Woo, E. J. (2018). Evaluation of hepatoprotective effect of curcumin on liver cirrhosis using a combination of biochemical analysis and magnetic resonance-based electrical conductivity imaging. Mediators of Inflammation, 2018, 1–9.
Lee, M. (2009). Basic skills in interpreting laboratory data (4th Ed.). American Society of Health-System Pharmacists.
Liang, Y. C., Tsai, S. H., Tsai, D. C., Lin-Shiau, S. Y., & Lin, J. K. (2001). Suppression of inducible cyclooxygenase and nitric oxide synthase through activation of peroxisome proliferator-activated receptor-gamma by flavonoids in mouse macrophages. FEBS Letters, 496(1), 12–18.
Lieberman, M. (1999). A brine shrimp bioassay for measuring toxicity and remediation of chemicals. Journal of Chemical Education, 76(12), 1689.
Manautou, J. E., Emeigh Hart, S. G., Khairallah, E. A., & Cohen, S. D. (1996). Protection against acetaminophen hepatotoxicity by a single dose of clofibrate: effects on selective protein arylation and glutathione depletion. Fundamental and Applied Toxicology: Official Journal of the Society of Toxicology, 29(2), 229–237.
Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E., & McLaughlin, J. L. (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica, 45(5), 31–34.
Miethke, M., Pieroni, M., Weber, T., Brönstrup, M., Hammann, P., Halby, L., ... & Müller, R. (2021). Towards the sustainable discovery and development of new antibiotics. Nature Reviews Chemistry, 5(10), 726–749.
Mohtasheem, M., Ahmad, S. W., Iqbal, A., & Ali, M. S. (2001). Brine shrimp bioassay of phoenix sylvestris. Pakistan Journal of Pharmaceutical Sciences, 14(2), 19–21.
Mujeeb, M., Aeri, V., Bagri, P., & Khan, S. (2009). Hepatoprotective activity of the methanolic extract of Tylophora indica (Burm. f.) Merill. leaves. International Journal of Green Pharmacy, 3(2), 125–127.
Nasim, N., Sandeep, I. S., & Mohanty, S. (2022). Plant-derived natural products for drug discovery: current approaches and prospects. The Nucleus: an international journal of cytology and allied topics, 65(3), 399–411.
Newman, D. J., & Cragg, G. M. (2020). Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. Journal of natural products, 83(3), 770–803.
Nirmala, M. J., & Durai, L. (2019). Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system. International Journal of Nanomedicine, 14, 6439–6450.
Otang, M. (2013). Assessment of potential toxicity of three South African medicinal plants using the brine shrimp (Artemia salina) assay. African Journal of Pharmacy and Pharmacology, 7(20), 1272–1279.
Parmar, S. R., Vashrambhai, P. H., & Kalia, K. (2010). Hepatoprotective activity of some plants extract against paracetamol induced hepatotoxicity in rats. Journal of Herbal Medicine and Toxicology, 4(2), 101–106.
Pithayanukul, P., Nithitanakool, S., & Bavovada, R. (2009). Hepatoprotective potential of extracts from seeds of Areca catechu and nutgalls of Quercus infectoria. Molecules, 14(12), 4987–5000.
Poole, A., & Leslie, G. B. (1989). A practical approach to toxicological investigations. Cambridge, England, Cambridge University Press.
Porchezhian, E., & Ansari, S. H. (2005). Hepatoprotective activity of Abutilon indicum on experimental liver damage in rats. Phytomedicine: International Journal of Phytotherapy and Phytopharmacology, 12(1-2), 62–64.
Prasad, P. P., Chakraborty, M. A. I. N. A. K., Haldar, S. A. G. N. I. K., Majumder, P. O. U. L. A. M. I., & Haldar, P. K. (2015). Evaluation of anti-cancer potential of methanol extract of Curcuma zedoaria. Asian Journal of Pharmaceutical and Clinical Research, 7(5), 309–313.
Rahman, M. H., Roy, B., Chowdhury, G. M., Hasan, A., & Saimun, M. S. R. (2022). Medicinal plant sources and traditional healthcare practices of forest-dependent communities in and around Chunati Wildlife Sanctuary in southeastern Bangladesh. Environmental Sustainability, 5(2), 207–241.
Rahmatullah, M., Mukti, I. J., Haque, A., Mollik, M., Parvin, K., Jahan, R., Chowdhury, M. H., & Rahman, T. (2009). An ethnobotanical survey and pharmacological evaluation of medicinal plants used by the Garo tribal community living in Netrakona district, Bangladesh. American Eurasian Network for Scientific Information, 3(3), 402–418.
Rashid, M. M. O., Ferdous, J., Banik, S., Islam, M. R., Uddin, A. H. M. M., & Robel, F. N. (2016). Anthelmintic activity of silver-extract nanoparticles synthesized from the combination of silver nanoparticles and M. charantia fruit extract. BMC Complementary and Alternative Medicine, 16(1), 242.
Reddy, K. P., Bid, H. K., Nayak, V. L., Chaudhary, P., Chaturvedi, J. P., Arya, K. R., Konwar, R., & Narender, T. (2009). In vitro and in vivo anticancer activity of 2-deacetoxytaxinine J and synthesis of novel taxoids and their in vitro anticancer activity. European Journal of Medicinal Chemistry, 44(10), 3947–3953.
Rumzhum, N. N., Rahman, M. M., Islam, M. S., Chowdhury, S. A., Sultana, R., & Parvin, M. N. (2008). Cytotoxicity and antioxidant activity of extractives from Mirabilis jalapa. Stamford Journal of Pharmaceutical Sciences, 1(1), 85–88.
Sandhar, H. K., Kumar, B., Prasher, S., Tiwari, P., Salhan, M., & Sharma, P. (2011). A review of phytochemistry and pharmacology of flavonoids. Internationale Pharmaceutica Sciencia, 1, 24–41.
Sari, S., Ginting, C. N., Chiuman, L., & Ginting, S. F. (2022). Effect of Ethanol Extract of White Turmeric (Curcuma Zedoaria) as Hepatoprotector in Male Rats Induced By CuSO4 Pentahydrate. Journal Research of Social Science, Economics, and Management, 1(6), 747–753.
Senthamilselvi, M. M., Kesavan, D., & Sulochana, N. (2012). An anti-inflammatory and anti-microbial flavone glycoside from flowers of Cleome viscosa. Organic and Medicinal Chemistry Letters, 2(19), 2–5.
Setyani, D. A., Rahayu, D. U. C., Handayani, S., & Sugita, P. (2020). Phytochemical and antiacne investigation of Indonesian white turmeric (Curcuma zedoaria) rhizomes. In IOP Conference Series: Materials Science and Engineering (Vol. 902, No. 1, p. 012066). IOP Publishing.
Silalahi, M. (2020). Curcuma zedoaria (Christm.) Roscoe: benefits and bioactivity. Eureka Herba Indonesia, 1(2), 41–48.
Shivaraj, G., Prakash, D., Vinayak, H., Avinash, M., Sonal, V., & Shruthi, K. (2009). A review on laboratory liver function tests. Pan African Medical Journal, 3(17), 17–29.
Sofowora, A., Ogunbodede, E., & Onayade, A. (2013). The role and place of medicinal plants in the strategies for disease prevention. African journal of traditional, complementary, and alternative medicines: AJTCAM, 10(5), 210–229.
Subositi, D., & Wahyono, S. (2019). Study of the genus Curcuma in Indonesia used as traditional herbal medicines. Biodiversitas Journal of Biological Diversity, 20(5), 1356–1361.
Sumarheni, S., Jauhari, J., Sam, A., Manggau, M. A., Nursyamsi, N., Aswad, M., & Sartini, S. (2023). Curcuma zedoaria Extract as a Potential Protective Agent against Doxorubicin-Induced Toxicities in Rats. Iranian Journal of Pharmaceutical Sciences, 19(3), 228–237.
Sumathi, S., Iswariya, G. T., Sivaprabha, B., Dharani, B., Radha, P., & Padma, P. R. (2013). Comparative study of radical scavenging activity and phytochemical analysis of fresh and dry rhizomes of Curcuma zedoaria. International Journal of Pharmaceutical Sciences and Research, 4(3), 1069.
Takate, S. B., Pokharkar, R. D., Chopade, V. V., & Gite, V. N. (2010). Hepato-protective activity of the ethyl acetate extract of Launaea intybacea (jacq) beauv in paracetamol induced hepato-toxicity in albino rats. International Journal of Pharmaceutical Sciences Review and Research, 1(2), 72–74.
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